HBB 2415: TRANSGENIC PLANTS AND ANIMALS (45 Contact Hours)a) Course PurposeThis course covers a wide range of plants and animals which have been transformed toaddress the challenges associated with food security and medicine including strategiesof gene transfer.b) Course ObjectivesAt the end of this course, the student should be able to;1. Describe the different transgenic animals and plants generated to address specificchallenges2. Describe the different strategies used in gene transfer3. Explain the concept of molecular pharming and the associated benefits.c) Course DescriptionWeek 1 Introduction to transgenic plants and animalsDefinitions, history, reasons for transformations, other methods formodificationWeek 2 Strategies for gene transfer :in animals and plants; Stem cells, oocytes, sperms, protoplastWeek 3 Methods of gene transferMicroinjection, electroporation, viral transferWeek 4 Methods of gene transferLeaf disc transformation, particle bombardment, agrobacterium mediatedgene transferWeek 5 CAT 1Week 6 Case studies of transgenic animals:Increased milk production, improved nutrition, improved growth rate in;cattle, fish, sheep and poultry. Improved leather and wool in sheep.Week 7 Case studies of transgenic plants:Pest resistance, Bt crops, viral, bacterial and fungal pest resistance,Week 8 Case studies of transgenic plants:Herbicide tolerance, delayed fruit ripening, increased oil content, improvednutrition and stress tolerance.Week 9 Plants and animals as biorectors in molecular pharming;production of pharmaceuticals, hormones, interferons and antibodies,Week 10 Plants and animals as biorectors in molecular pharming;Production of polyhydroxybutyrate for biodegradable plastics, cyclodextrinsin drug delivery, flavour and odourWeek 11 Plants and animals in molecular pharming;Production of edible vaccines, ethical issues associated with transgenicplant and animals, possible effects of transgenics, oral presentations ofclass assignmentsWeek 13 CAT IId) Teaching MethodologiesLectures, Assignments, Tutorials, Demonstrations, Case Studies, Class Presentations,Group discussions.e) Instructional Materials/ EquipmentsCourse notes, marker boards, markers, dusters, computer and LCD projector.Laboratory equipments, materials and reagents for practicals.f) Course AssessmentPracticals, 15%, Assignments 5%, CATS 10%, Semester Examinations 70%.g) Course Textbooks1. Miller, P. (2011). Emerging Trends in Antibacterial Discovery, Published by CaisterAcademic Press. ISBN: 978-1-904455-89-9.3. Stanbury, P. F., Allan, W. R. and Stephen, J. H. (1999). Principles of fermentationTechnology. 2nd Edition Butterworth Heinemann Publishers. ISBN 0750645016, 978-0-7506-4501-0.4. Gary, W. (2007). Pharmaceutical Biotechnology, Concepts and Application. JohnWiley and Sons Ltd ISBN 978-0-470-01244-4.h) Course Journals1. Journal of Biotechnology. Published by Elsevier Science. ISSN 0168-1656.2. International Journal of Biotechnology and Biochemistry. Published byResearch India. Print ISSN 0973-2691, Online ISSN 0974-4762.3. Current Opinion in Biotechnology. Published by Elsevier Science. ISSN 0958-1669.i) Further References1. Shirahata, S., Teruya, K. and Katakura, Y. (2010). Animal cell technology: Basic andApplied Aspects. Published by Springer. ISBN 978-90-481-3891-3.2. Nigel, J. (1999). Animal cell Biotechnology; methods and protocols. Published bySpringer. ISBN: 978-0-89603-547-8 (Print) 978-1-59259-486-3 (Online).3. Lindsey, K. and Jones, M.G.K. (2007). Plant Biotechnology in Agriculture. Published byOpen University Press. ISBN: 0335158188, 9780335158188.
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